Cost Structure Effective February 26, 2009

Internal Rates (BYU PIs Only)
$6.00 - Sequencing Charge (BigDye Rxn, Clean-up + Electrophoresis)
$0.50 - Lane Charge (Electrophoresis only)
$2.00 - PCR Purification
$0.45 - Sample Processing by the Parallab

External Rates
$9.00 - Sequencing Charge (BigDye Rxn, Clean-up + Electrophoresis)
$4.50 - Lane Charge (Electrophoresis only)
$1.50 - PCR Purification

Other Services
Fragment Analysis
Lane charge + $0.25 for addition of ROX 500 (or LIZ if you so prefer) Size Standard
(Lanes from standing order CAN be used)

Next generation DNA Sequencing
Submitting samples for next generation sequencing on the 454 Life Sciences DNA sequencing machine is now possible. Typically some 1.3 million beads pass the quality filters. You often end up with more than 500 million bases over the two halves of the plate. We hope to provide at least 400 million bases of DNA sequence information, but regardless of how many bases of DNA sequence that we return, the price is the same. There are many kits that are needed in setting up and then running a sample on the 454 Life Sciences machine. Rather than list all these components, we are for now stating that it will cost a minimum of $6,200 for a Titanium run. The cost for your run will likely be higher, talk to Ed Wilcox for more details. We can also perform the FLX runs (which generate 100 million bases of data instead of 500 million).

When will you get your 454 Life Science data?
Please be patient, this takes about a week once we have your DNA sample. We are always willing to talk with you about where we are in processing your sample.

Pricing for non-BYU faculty. The cost for a for a full library preparation and Titanium run is $9,300. If you are willing to do all the library and bead preparations, the cost is $6,200 for us to load and run the plate and send you a "Blue Ray" disk containing images and the data from the processed images.

Manuals detailing all the procedures involved in performing a 454 Life Sciences run are available at links posted on the "Protocols" section of this browser.

When will you get your ABI3730xl data?
If we have your samples before 5:00 PM, you will usually have your data the following morning, you may get it earlier depending on where your submission is in relation to other submissions. This same rule applies to Friday evening and Saturday morning. If you submit samples on Saturday, you will likely have your data Monday morning. If your samples are placed in our refrigerator after 5:00 PM, you may still get your data by the following morning.

DNASC users submitting samples for Cycle Sequencing are requested to have your samples in our refrigerator by noon of Tuesdays and Thursdays, you will usually have your data by the following morning.

ABI Billing Rates

For internal BYU investigators your billing rate is listed at the top of the page. For External users you will need to pre-purchase your sequencing plans using the schedule listed below.

Sequencing Plans include BigDye reaction, clean-up and electrophoresis. Purified DNA templates and primers need to be provided at the right concentration.
Lane Plans include only capillary electrophoresis. Users need to perform BigDye reaction and clean-up reactions at their own cost. If you haven't already dried the samples down before submission, we will do so here prior to running them.

Internal - Sequencing Plans PLAN SAMPLES PRICE/SPL TOTAL COST IS-1 1 $6.00 $6.00	External - Sequencing Plans PLAN SAMPLES PRICE/SPL TOTAL COST ES-0 1 $9.00 $9.00 ES-1 100 $8.68 $867.60 ES-2 200 $8.35 $1,670.40 ES-4 400 $7.83 $3,132.00 ES-6 600 $7.06 $4,233.60 ES-8 800 $6.41 $5,126.40 ES-10 1,000 $6.03 $6,030.00 ES-20 2,000 $5.58 $11,160.00 ES-40 4,000 $5.40 $21,600.00 ES-60 6,000 $5.22 $31,320.00
Internal - Lane Plans PLAN SAMPLES PRICE/SPL TOTAL COST IL-1 1 $0.50 $0.50	External - Lane Plans PLAN SAMPLES PRICE/SPL TOTAL COST EL-0 1 $4.50 $4.50 EL-1 100 $4.32 $432.00 EL-2 200 $4.14 $828.00 EL-3 300 $3.96 $1,188.00 EL-4 400 $3.78 $1,512.00 EL-6 600 $3.42 $2,052.00 EL-8 800 $3.24 $2,592.00 EL-10 1,000 $3.06 $3,060.00
Internal - PCR Purification Plans PLAN SAMPLES PRICE/SPL TOTAL COST IPC-1 1 $2.00 $2.00	External - PCR Purification Plans PLAN SAMPLES PRICE/SPL TOTAL COST EPC-0 1 $2.00 $2.00 EPC-1 100 $1.35 $135.00 EPC-3 300 $1.26 $378.00 EPC-5 500 $1.17 $585.00 EPC-10 1,000 $1.05 $1,050.00
Internal - Parallab350 PLAN SAMPLES PRICE/SPL TOTAL COST IPara 380 $0.45 $171.00

We have established a standing order purchase policy for external users in order to provide a more economical option for high volume external users.

Additional Explanations and Policies

The internal rates are for those PIs who are directly associated with BYU as faculty, staff, or student. All others are considered external users.

Lane charges refer to samples cycle sequenced and dried by the user before submission to the DNASC; Sequencing charges apply to clones or PCR products sent to be cycle sequenced by the facility.

We run positive controls with every sequencing and fragment analysis run. If we are unable to obtain quality sequence from our control, as well as from your DNA, you will not be assessed a charge or we will rerun your samples. We want you to be satisfied with the data we provide to you.

Billing is monthly and is based on the charges incurred from the 26th of the previous month to the 25th of the current month.

For more details or other questions please contact Dr. Edward Wilcox, Manager, DNASC. Phone:(801) 422-3647

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Policies

Samples

• Dried samples, already cycle sequenced, ready for electrophoresis
• Individual samples (up to 31) must be in 1.7 ml microfuge tubes. Clearly label each tube numerically (1, 2, 3, etc) or alphanumerically (J-1, J-2, etc) on the lid. Long names written on the tubes create confusion and are not acceptable.
• Batches of 32 samples or more must be submitted in 96-well V bottom microplates or PCR plates, dried and covered with adhesive plate seal.
• Samples on 96-well plates will be loaded by column following the order: A1, B1 to H1, A2, B2 to H2 and so forth until H12. Your samples need to be submitted in this same order on your submitted plate. Follow the same format for partially filled plates and cross off any empty wells.

Fragment Analysis Samples

• We run Fragment Analysis/Gene Scan samples in batches of 16 up to 96 lanes.
• Samples need to be in 96-well PCR plates.
• Samples on 96-well plates will be loaded by column following the order: A1, B1 to H1, A2, B2 so forth until H12. Your samples need to be in that order on your plate. Follow the same format for partially filled plates and cross off any empty wells.

Purified PCR and plasmids submitted for cycle sequencing by the DNASC

• Samples need to be in 1.7 ml microfuge tubes, 96-well V microplates or PCR plates (32 samples or more).
• We need a volume of at least 4 µl.
• Primer must already be added to the sample (about 1.1 pmoles per µl, one primer per tube). The facility does not provide primers to users.
• Again, clearly label the tubes numerically or alphanumerically on the lid.

Sample brought in for post-PCR cleaning prior to cycle sequencing

• We currently use Exonuclease I/Antarctic Phosphatase to process these samples
  
• At least 10 µl of PCR reaction needs to be submitted for processing.
• Primers need to be aliquoted into individual tubes at a 3.2 µM concentration. We need 2 µl of primer per sample.

DNASC policies

• The tray with your samples needs to have your name and submission code.
• Your samples will be kept for two months from the arrival date.
• Electrophoresed samples will be stored @ -20 °C for 20 days. Reruns need to be requested ASAP.
  

Server

• Zip files will be available on the DNASC server for download for 45 days. After 45 days, all data is stored on DVD. Users are responsible for backing up their own sequence data.
• All users are required to submit sample information on line. Samples without an on line submission cannot be processed.

DNASC Work Schedule

• Samples will be electrophoresed according to submission order but very large submissions (more than five 96 well plates) will be processed gradually in order to accommodate the needs and wants of smaller users.
• The DNASC is closed Saturdays, Sundays and major holidays.

Please contact the DNASC if you have any questions about our policies.